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cell culture immortalized human ectocervical cells  (ATCC)


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    Structured Review

    ATCC cell culture immortalized human ectocervical cells
    Human <t>ectocervical</t> cells <t>(ECT1)</t> were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.
    Cell Culture Immortalized Human Ectocervical Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture immortalized human ectocervical cells/product/ATCC
    Average 96 stars, based on 342 article reviews
    cell culture immortalized human ectocervical cells - by Bioz Stars, 2026-05
    96/100 stars

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    1) Product Images from "Viral infection of the pregnant cervix predisposes to ascending bacterial infection"

    Article Title: Viral infection of the pregnant cervix predisposes to ascending bacterial infection

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1300661

    Human ectocervical cells (ECT1) were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.
    Figure Legend Snippet: Human ectocervical cells (ECT1) were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.

    Techniques Used: Blocking Assay, Infection, Western Blot



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    96
    ATCC cell culture immortalized human ectocervical cells
    Human <t>ectocervical</t> cells <t>(ECT1)</t> were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.
    Cell Culture Immortalized Human Ectocervical Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture immortalized human ectocervical cells/product/ATCC
    Average 96 stars, based on 1 article reviews
    cell culture immortalized human ectocervical cells - by Bioz Stars, 2026-05
    96/100 stars
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    Human ectocervical cells (ECT1) were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Viral infection of the pregnant cervix predisposes to ascending bacterial infection

    doi: 10.4049/jimmunol.1300661

    Figure Lengend Snippet: Human ectocervical cells (ECT1) were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.

    Article Snippet: Cell culture Immortalized human ectocervical cells (ECT1, ATCC, CRL-2614) were cultured in keratinocyte serum free medium (17005-042, Gibco, Grand Island, NY) with bovine pituitary extract and hEGF supplementation as recommended by ATCC under 5% CO2 at 37°C.

    Techniques: Blocking Assay, Infection, Western Blot